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Published on: August 4, 2021
Genetically Encodable Fluorescent and Bioluminescent Biosensors Light Up Signaling Networks
Xin Zhou1, Sohum Mehta1, Jin Zhang1
1Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Abstract:
Cell signaling networks are intricately regulated in time and space to determine the responses and fates of cells to different cues. Genetically encodable fluorescent and bioluminescent biosensors enable the direct visualization of these spatiotemporal signaling dynamics within the native biological context, and have therefore become powerful molecular tools whose unique benefits are being used to address challenging biological questions. We first review the basis of biosensor design and remark on recent technologies that are accelerating biosensor development. We then discuss a few of the latest advances in the development and application of genetically encodable fluorescent and bioluminescent biosensors that have led to scientific or technological breakthroughs.
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