Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats

This study aimed to explore a valid test protocol for measuring VO_2max in healthy untrained male Wistar rats of different ages and quantifying the exercise intensity (%VO_2max) of running under different treadmill grades and speeds. The test protocols and %VO_2max will provide a reference for the design of exercise intensity. We tested male Wistar rats aged 4 weeks, 10 weeks, 10 months and 16 months old with three test protocols (Procedure 1 [P1], 2 [P2] and 3 [P3]) for each age group to quantify VO_2max. We analysed VO_2max, respiratory exchange ratio and test duration to determine an optimal test protocol of VO_2max for different age groups. We used the optimal test protocol to explore the changes in age-related VO_2max. Finally, %VO_2max of running under different treadmill speeds and grades was quantified. VO_2max of Wistar rats decreased significantly after the age of 4 weeks (p < 0.05). The optimum VO_2max can be induced by personalised protocols for different ages. In 4-week-old Wistar rats, the highest VO_2max values were attained by P1 (104.4 ± 6.9 mL · kg^-1 · min^-1, p = 0.032). The highest VO_2max value (84.7 ± 3.7 mL · kg^-1 · min^-1, p = 0.037) of 8-week-old Wistar rats was attained in P2. In 10-month-old Wistar rats, the highest VO_2max value was obtained in P3 (63.3 ± 1.7 mL · kg^-1 · min^-1). This work could be used as a reference for assessing aerobic capacity in studies on exercise intervention with untrained male Wistar rats. However, the %VO_2max measurements at various treadmill speeds and grades only apply to untrained male Wistar rats.