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Published on: March 2, 2014
Changes in inflammatory factors in SV40MES13 mesangial cells after silencing ApoM gene
Jiongjiong Liu1, Yang Zhang2, Xiao Zhang3
1Department of International Medical Services, The First Affiliated Hospital of USTC-Divsion of Life Sciences and Medicine, University of Sciences and Technology of China Hefei 230001, China.
Objective:
Inflammation is an important process in the occurrence and development of nephropathy, and ApoM is closely related to inflammation. This article aims to investigate the inflammatory changes of SV40 MES13 cells after ApoM gene silencing by western blot and to explore the relationship between ApoM and inflammation.
Methods:
Control group glomerular mesangial cells (SV40 MES13), and the same cells after adding a small interfering RNA silencing ApoM gene for 24 h were observed under a microscope and photographed. After extracting the protein western blot was used to explore the associated inflammation of IL-6, P-Jak2, Erk, TNF-α, P-JNK, IKKβ, P-p38, IκBα, P-IKKα/β, NF-κB and P-NF-κB expression.
Results:
Western blot showed that ApoM gene was successfully silenced in SV40 MES13 cells after adding small interfering RNA. The decrease of inflammatory factors IL-6 and P-Jak2 in Jak/Stat pathway was statistically significant. Inflammatory cytokines TNF-α and P-JNK in the NF-κB pathway decreased statistically significantly, while the inflammatory factor IKKβ increased statistically significantly.
Conclusion:
Inflammation was suppressed in SV40 cells with ApoM gene silencing.
Insights
Gene silencing of Apolipoprotein M (ApoM) suppressed inflammation in kidney cells. This study investigated inflammatory markers in SV40 MES13 cells following ApoM gene silencing, revealing significant reductions in key inflammatory factors.
Area of Science:
- Nephrology
- Molecular Biology
- Immunology
Background:
- Inflammation plays a critical role in the development and progression of nephropathy.
- Apolipoprotein M (ApoM) has been implicated in inflammatory processes.
Purpose of the Study:
- To investigate the impact of ApoM gene silencing on inflammatory changes in SV40 MES13 cells.
- To explore the relationship between ApoM and cellular inflammation.
Main Methods:
- SV40 MES13 cells were treated with small interfering RNA to silence the ApoM gene.
- Protein expression of inflammatory markers (IL-6, TNF-α, P-Jak2, P-JNK, IKKβ, P-p38, IκBα, NF-κB) was analyzed using Western blot.
Main Results:
- Successful silencing of the ApoM gene was confirmed via Western blot.
- Significant decreases in inflammatory factors IL-6 and P-Jak2 (Jak/Stat pathway) were observed.
- Statistically significant reductions in TNF-α and P-JNK (NF-κB pathway) were noted, alongside a significant increase in IKKβ.
Conclusions:
- ApoM gene silencing effectively suppressed inflammation in SV40 MES13 cells.
- These findings suggest ApoM is a potential therapeutic target for inflammatory kidney diseases.
