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Dynamics of Hepatitis B Virus Capsid Protein Dimer Regulate Assembly through an Allosteric Network
Angela Patterson1, Zhongchao Zhao2, Elizabeth Waymire1
1Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, United States.
ACS Chemical Biology
|July 15, 2020
Summary
Protein dynamics control Hepatitis B Virus (HBV) capsid assembly. Understanding this process, including the role of dimer building blocks and mutations, offers potential therapeutic targets for chronic HBV infections.
Area of Science:
- Biochemistry
- Structural Biology
- Virology
Background:
- Chronic Hepatitis B Virus (HBV) infection affects millions globally, with no cure.
- The viral capsid assembly is a key target for therapeutic intervention.
- Understanding the dynamics of capsid protein dimers is crucial for deciphering assembly mechanisms.
Purpose of the Study:
- To investigate the role of protein dynamics in Hepatitis B Virus (HBV) capsid assembly.
- To identify key structural and dynamic features regulating capsid formation using mutant and homologous proteins.
- To map an allosteric network within the HBV dimer that influences assembly.
Main Methods:
- Hydrogen-deuterium exchange mass spectrometry (HDX-MS) to measure protein dynamics.
- Analysis of an assembly-incompetent HBV capsid protein mutant (Cp149-Y132A).
- Comparative studies using woodchuck Hepatitis B Virus (WHV) capsid protein (Cp) for stability and assembly rate insights.
- Native mass spectrometry.
Main Results:
- A mutation in the HBV dimer (Cp149-Y132A) altered protein dynamics, impacting assembly.
- Woodchuck HBV (WHV) capsid protein showed greater stability and faster assembly compared to human HBV.
- Comparative dynamic analysis revealed an allosteric network within the HBV dimer.
Conclusions:
- Protein subunit dynamics are critical regulators of Hepatitis B Virus (HBV) capsid assembly.
- Differences in stability and dynamics between HBV and WHV Cps highlight conserved and divergent assembly mechanisms.
- Targeting protein dynamics offers a potential strategy for developing new HBV therapies.
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