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Standardization procedure for flow cytometry data harmonization in prospective multicenter studies.
Lucas Le Lann1, Pierre-Emmanuel Jouve2, Marta Alarcón-Riquelme3
1INSERM, UMR1227, CHRU Morvan, Lymphocytes B et Autoimmunité, Univ Brest, BP 824, 29609, Brest, France.
Scientific Reports
|July 16, 2020
Summary
Standardizing clinical cytometry data is crucial for multicenter trials. This workflow enables reliable comparison of leukocyte populations and marker intensities across diverse instruments, facilitating large-scale immunomonitoring projects.
Area of Science:
- Clinical cytometry
- Immunology
- Biomedical instrumentation
Background:
- Comparing flow cytometry data across multiple centers and instruments presents significant challenges.
- Variability in instrument performance and data acquisition protocols can compromise the accuracy of immunomonitoring trial results.
Purpose of the Study:
- To develop and present an innovative standardization workflow for clinical cytometry.
- To enable robust comparison of leukocyte populations and cell marker expression in prospective multicenter studies.
Main Methods:
- Implementation of a novel standardization workflow designed for flow cytometry data.
- The workflow facilitates data comparison regardless of instrument type, number, or project duration.
Main Results:
- The described workflow allows for the reliable comparison of cell frequencies, absolute leukocyte numbers, and mean fluorescence intensities.
- Enables consistent analysis of flow cytometry data generated from different instruments.
Conclusions:
- This standardization workflow addresses a key challenge in clinical cytometry for multicenter trials.
- Facilitates large-scale, prospective immunomonitoring analyses with comparable data across diverse settings.
