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Antibodies to a short synthetic peptide cross-react with human recombinant interleukin 1 alpha
M E Davies1, C G Knight, B Y Mativi
1Tissue Physiology Department, Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
Immunology Letters
|December 1, 1988
Abstract:
An antiserum to human interleukin 1 alpha has been prepared by immunizing a sheep with a short synthetic peptide (Mr 1427) conjugated to keyhole limpet haemocyanin using the heterobifunctional cross-linking agent N-succinimidyl bromoacetate. The peptide was selected from a highly hydrophilic region corresponding to residues 169-179 of the cDNA-derived sequence. Two additional peptides corresponding to residues 194-207 and 224-233 failed to elicit cross-reacting antibodies.