Long-term STED imaging of amyloid fibers with exchangeable Thioflavin T
Joaquim Torra1, Patricia Bondia1, Sylvia Gutierrez-Erlandsson2
1Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanociencia), Madrid, Spain. cristina.flors@imdea.org.
Nanoscale
|July 16, 2020
Abstract:
We report the use of the amyloid probe Thioflavin T (ThT) as a specific and exchangeable fluorophore for stimulated emission depletion (STED) super-resolution imaging of amyloid fibers. This method achieves a spatial resolution in the range of 60-70 nm, low image background and increased photostability that enables long-term STED imaging. These results expand the widespread uses of ThT and can be potentially extended to other common amyloid fluorescent probes, providing new tools for the study of amyloid diseases.
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