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Updated: Dec 14, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multi-line FLEET by imaging periodic masks.

Yibin Zhang, Garrett Marshall, Steven J Beresh

|July 16, 2020

Summary

A new multi-line femtosecond laser electronic excitation tagging (FLEET) velocimetry method images supersonic jets. This technique enhances spatial coverage for flowfield analysis in short-duration facilities.

Area of Science:

Fluid Dynamics
Optical Diagnostics

Background:

Supersonic jet research requires advanced flow visualization techniques.
Traditional velocimetry methods may lack sufficient spatial coverage for complex flows.

Purpose of the Study:

To introduce and validate a novel multi-line femtosecond laser electronic excitation tagging (FLEET) velocimetry configuration.
To apply this method for imaging an overexpanded unsteady supersonic jet.

Main Methods:

A simple linear configuration using periodic masks to generate multiple FLEET lines (0.5-1.0 mm spacing).
Simultaneous imaging of up to six FLEET lines.
Calculation of mean velocity and turbulence intensity maps.

Main Results:

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Last Updated: Dec 14, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

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Successful imaging of an overexpanded unsteady supersonic jet using multi-line FLEET.

Computed velocities show good agreement with traditional single-line FLEET.

Demonstrated increased simultaneous spatial coverage compared to traditional FLEET.

Conclusions:

Multi-line FLEET provides enhanced spatial coverage for flowfield analysis.
This technique is suitable for studying unsteady supersonic jets, particularly in short-duration test facilities.
Enables spatial correlation measurements in the streamwise direction.