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A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
Published on: February 17, 2023
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EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS
Letícia Fuganti Campos1, Eliane Tagliari1, Thais Andrade Costa Casagrande2
1Postgraduate Program in Surgical Clinic, Federal University of Paraná, Curitiba PR, Brazil.
Summary
Probiotic supplementation significantly enhanced skin wound healing in diabetic rats by accelerating contraction and increasing collagen deposition. This suggests probiotics may be a viable therapeutic strategy for chronic wound management.
Area of Science:
- Wound healing research
- Diabetic complications
- Microbiome modulation
Background:
- Chronic wounds in Diabetes Mellitus are challenging due to excessive inflammatory cytokines.
- Probiotics can modify gut microbiota and modulate inflammatory responses.
Purpose of the Study:
- To investigate the effect of perioperative probiotic supplementation on cutaneous wound healing in diabetic rats.
Main Methods:
- Diabetic rats were divided into control and probiotic groups, receiving oral administration.
- Wounds were induced and left to heal by second intention, with measurements taken on postoperative days 3 and 10.
- Collagen deposition and histological parameters were analyzed.
Main Results:
- Probiotic groups showed faster wound contraction and smaller scar areas by day 10.
- Increased type I collagen deposition was observed in probiotic groups from day 3 to day 10.
- Histological analysis revealed improved healing, reduced inflammation, and increased neovascularization in probiotic-treated rats.
Conclusions:
- Perioperative probiotic supplementation promotes skin wound healing in diabetic rats.
- This effect is likely mediated by reduced inflammation and enhanced collagen synthesis and neovascularization.
