Mucilaginibacter agri sp. nov. and Mucilaginibacter humi sp. nov., isolated from soil

Two Gram-stain-negative, facultative anaerobic and non-motile bacteria, strains R11^T and S1162^T, were isolated from soil in the Republic of Korea. Both strains were catalase- and oxidase-positive and contained menaquinone-7 as the major isoprenoid quinone. Strain R11^T contained summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), iso-C_15:0, C_16:0 and iso-C_17:0 3-OH as major fatty acids and phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified aminolipid as major polar lipids; while strain S1162^T contained summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), iso-C_15:0, iso-C_17:0 3-OH, C_16:0 and summed feature 9 (10-methyl C_16:0 and/or iso-C_17:1 ω9c) as major fatty acids and phosphatidylethanolamine and an unidentified aminophospholipid as major polar lipids. The DNA G+C contents of strains R11^T and S1162^T calculated from their whole genomes were 42.7 and 42.9 mol%, respectively. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain R11^T formed a phylogenetic lineage with Mucilaginibacter jinjuensis YC7004^T and strain S1162^T formed a distinct phyletic lineage from closely related type strains within the genus Mucilaginibacter. Strains R11^T and S1162^T were most closely related to M. jinjuensis YC7004^T and Mucilaginibacter panaciglaebae BXN5-31^T with 97.78 and 97.23% 16S rRNA gene sequence similarities, respectively. On the basis of phenotypic, chemotaxonomic and molecular analysis, strains R11^T and S1162^T represent two novel species of the genus Mucilaginibacter, for which the names Mucilaginibacter agri sp. nov. and Mucilaginibacter humi sp. nov. are proposed, respectively. The type strains of M. agri and M. humi are R11^T (=KACC 21228^T=JCM 33472^T) and S1162^T (=KACC 21669^T=JCM 33916^T), respectively.