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Updated: Dec 14, 2025
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Start-up of single-stage partial nitritation-anammox micro-granules system: Performance and microbial community
Shaopo Wang1, Jianyu Li1, Dong Wang1
1School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26, Tianjin, 300384, China; Tianjin Key Laboratory of Aquatic Science and Technology, Jinjing Road 26, Tianjin, China; Joint Research Centre for Protective Infrastructure Technology and Environmental Green Bioprocess, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, China.
Abstract:
By manipulating influent nitrogen load and DO concentration in bulk liquid, the start-up and performance of a new micro-granule based partial nitritation-anammox process was investigated in a continuous stirred tank reactor (CSTR). Under the condition of nitrogen loadings from 0.3 to 1.4 kgN /m³/d and DO <0.21mg/L, the single-stage partial nitritation-anammox (SPNA) system was successfully started, with a nitrogen removal of 76.2%. Meanwhile, the oxygen utilization efficiency by ammonium oxidizing bacteria (AOB) increased in the system with the increase of influent ammonia loading rate. Micro-granules with an average diameter of 0.25 mm were formed. Sludge granulation was promoted by increasing influent nitrogen load, and there was a positive correlation between nitrogen load, extracellular polymeric substances (EPS) content and sludge particle size. Ca. Kuenenia became the dominant anaerobic ammonium oxidizing bacteria (AnAOB) in the SPNA system. As the dominant AOB genera, Nitrosomonas coexist with Ca. Kuenenia in the micro-granules.
