[The immunohistological study of the lymphoid malignancy using cell surface or differentiating markers and cell...

This research was the study of hemato-malignancy to clarify their cell origin and biological character by 14 monoclonal antibodies, which were 13 antibodies as surface or differentiate markers and Ki-67 as cell proliferating nuclear marker. Ki-67 recognizes PC-antigen in nuclear. 23 cases of lymphoid malignancy were selected out of 104 cases of hemato-lymphoid disorders, which were consulted to the second department of pathology of Hokkaidou University for marker study or histologic investigation. 21 cases was malignant lymphoma (primary biopsy, 18 cases non-Hodgkin's lymphomas, 3 cases Hodgkin's lymphoma), And 2 cases were myeloma (one autopsy case, multiple myeloma; another biopsy case, solitary myeloma of the thoracic bone). By the result of this study, the strong correlation was found the expression of surface or differentiate markers between the cyto-histomorphology of the lymphoid malignancy. Especially, the result of Ki-67 did clarify more vividly the biological character of these lymphoid malignancy than that of surface or differentiate markers. The results obtained by western-blotting and immuno-electron microscopy of Ki-67 showed that PC-antigen was one of the preribosomal granules in granular component of nucleolar and it's MW was about 30 KD. I discussed this point from the morphological aspect in this paper and concluded that the fact which PC-antigen was one of preribosomal granules is the reason of Ki-67 serving as the cell proliferating nuclear marker.