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Updated: Dec 14, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO
Zhenyu Li1,2, Yun Feng2, Zhen Zhang2
1Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhongyuan District, Zhengzhou, 450000 Henan China.
Background:
Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulatory mechanism of TMPO-AS1 in TC.
Methods:
TMPO-AS1 and TMPO expression in TC tumors and cells was detected by TCGA database and QRT-PCR assay respectively. CCK-8, EDU, TUNEL and western blot assays were conducted to identify the biological functions of TMPO-AS1 in TC. Luciferase reporter and RNA pull down assays were conducted to measure the interaction among TMPO-AS1, TMPO and miR-498.
Results:
TMPO-AS1 was overexpressed in TC tissues and cell lines. Knockdown of TMPO-AS1 suppressed cell growth and accelerated cell apoptosis in TC. Furthermore, downregulation of TMPO-AS1 suppressed TMPO expression in TC. The data suggested that TMPO expression was upregulated in TC tissues and cell lines and was positively correlated with TMPO-AS1 expression in TC. Furthermore, the expression of miR-498 presented low expression in TC cells. And miR-498 expression was negatively regulated by TMPO-AS1, meanwhile, TMPO expression was negatively regulated by miR-498 in TC cells. Besides, it was confirmed that TMPO-AS1 could bind with miR-498 and TMPO in TC cells. In addition, it was validated that TMPO-AS1 elevated the levels of TMPO via sponging miR-498 in TC cells.
Conclusions:
TMPO-AS1 promotes cell proliferation in TC via sponging miR-498 to modulate TMPO.
Insights
Long noncoding RNA TMPO-AS1 promotes thyroid cancer (TC) cell proliferation. It acts by sponging miR-498 to modulate TMPO expression, offering a potential therapeutic target for this common endocrine malignancy.
Area of Science:
- Endocrinology
- Molecular Biology
- Oncology
Background:
- Thyroid cancer (TC) is the most common endocrine malignancy.
- Long noncoding RNAs (lncRNAs) play crucial roles in tumor development.
- The specific role of lncRNA TMPO-AS1 in TC remains largely unexplored.
Purpose of the Study:
- To investigate the role of lncRNA TMPO-AS1 in thyroid cancer.
- To elucidate the regulatory mechanism of TMPO-AS1 in TC progression.
Main Methods:
- Expression analysis of TMPO-AS1 and TMPO in TC tissues and cells using TCGA and QRT-PCR.
- Functional assays including CCK-8, EDU, TUNEL, and western blot to assess TMPO-AS1's biological functions.
- Luciferase reporter and RNA pull-down assays to determine interactions between TMPO-AS1, TMPO, and miR-498.
Main Results:
- TMPO-AS1 was significantly overexpressed in TC tissues and cell lines.
- Knockdown of TMPO-AS1 inhibited TC cell proliferation and induced apoptosis.
- TMPO-AS1 positively correlated with TMPO expression and negatively regulated miR-498, while miR-498 negatively regulated TMPO.
- TMPO-AS1 promotes TMPO expression by sponging miR-498 in TC cells.
Conclusions:
- TMPO-AS1 acts as an oncogenic lncRNA in thyroid cancer.
- TMPO-AS1 promotes TC cell proliferation through the TMPO-AS1/miR-498/TMPO axis.
- Targeting TMPO-AS1 may represent a novel therapeutic strategy for thyroid cancer.
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