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Related Experiment Video

Updated: Dec 14, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

Proposal for Coronavirus Disease 2019 Management.

John B Downs^1,2, Barry Weled³, Jukka Räsänen⁴

¹Departments of Anesthesiology and Critical Care Medicine, University of Florida College of Medicine, Gainesville, FL.

Critical Care Explorations

|July 17, 2020

Summary

This summary is machine-generated.

Early use of facemask continuous positive airway pressure (CPAP) for COVID-19 patients may reduce the need for mechanical ventilators. This approach conserves resources and protects healthcare workers during pandemics.

Keywords:

continuous positive airway pressure coronavirus disease 2019 hypoxemia

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Remote Laboratory Management: Respiratory Virus Diagnostics

Remote Laboratory Management: Respiratory Virus Diagnostics

Published on: April 6, 2019

Area of Science:

Critical Care Medicine
Infectious Disease Epidemiology
Respiratory Medicine

Background:

The coronavirus disease 2019 (COVID-19) pandemic created widespread fear and predictions of critical resource shortages, including ventilators.
Patients with COVID-19 often present with early respiratory distress and hypoxemia, but typically not hypercapnia.

Purpose of the Study:

To evaluate the efficacy of non-invasive ventilation strategies in managing COVID-19 patients.
To determine if early intervention with continuous positive airway pressure (CPAP) can prevent the need for mechanical ventilation.
To assess the impact of CPAP on protecting healthcare workers from viral transmission.

Main Methods:

Utilized facemask continuous positive airway pressure (CPAP) as an intervention for patients with COVID-19 experiencing respiratory distress.
Applied CPAP protocols designed to maintain adequate alveolar ventilation, normocapnia, and oxygenation.
Implemented safety protocols to protect healthcare providers from aerosolized infectious particles.

Main Results:

Facemask CPAP, when applied early and titrated effectively, demonstrated potential to avoid tracheal intubation in COVID-19 patients.
The use of CPAP facilitated spontaneous ventilation, reducing the need for sedatives and paralytics.
Protocols were successful in preventing viral transmission to caregivers.

Conclusions:

Early application of facemask CPAP is a viable strategy to conserve mechanical ventilators during pandemics.
This non-invasive approach optimizes resource allocation and protects healthcare personnel.
CPAP allows for spontaneous breathing, potentially decreasing the demand for specialized staff and intensive care unit resources.