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Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins
J A McKenzie1, R L Raison, D E Rivett
1Clinical Immunology Research Centre, University of Sydney, New South Wales, Australia.
Journal of Protein Chemistry
|October 1, 1988
Abstract:
A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of 14C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins.