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Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans
Diana Alves1,2, Rodrigo Curvello1,2, Edward Henderson1,2,3
1Department of Chemical Engineering, ARC Centre of Excellence in Convergent BioNano Science and Technology, Monash University, Clayton, Victoria 3800, Australia.
ACS Sensors
|July 17, 2020
Summary
Rapid agglutination assays using blood typing methods can quickly detect SARS-CoV-2 antibodies. This high-throughput approach aids in COVID-19 case identification and population screening.
Area of Science:
- Immunology
- Virology
- Biotechnology
Background:
- Accurate serology assays for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) are crucial for understanding COVID-19's global impact.
- Existing needs include rapid case identification, contact tracing, and population screening to control viral spread.
Purpose of the Study:
- To adapt agglutination tests, commonly used in blood typing, for high-throughput SARS-CoV-2 serology.
- To develop a rapid, scalable, and cost-effective assay for detecting SARS-CoV-2 antibodies.
Main Methods:
- Utilized column agglutination testing combined with peptide-antibody bioconjugates.
- Designed bioconjugates to facilitate red blood cell cross-linking in the presence of SARS-CoV-2 specific antibodies.
Main Results:
- Demonstrated the feasibility of using agglutination assays for SARS-CoV-2 serological testing.
- The developed method is rapid (10-30 minutes) and aligns with ASSURED criteria for diagnostic assays.
Conclusions:
- Agglutination assays offer a simple, fast, and scalable solution for SARS-CoV-2 serological testing.
- This platform has immediate applications for COVID-19 and potential for future assay development.
