Improving CH₄ production and energy conversion from CO₂ and H₂ feedstock gases with mixed methanogenic community over...

To achieve methanogenic community optimization and improve the conversion efficiency of CO₂ to CH₄, Fe nanoparticles were used to promote the Methanothermobacter abundance in methanogens, which significantly increased the conversion efficiency of CO₂ and H₂ feedstock gases to CH₄ product. High-throughput 16S rRNA gene sequencing analysis revealed that Methanothermobacter abundance markedly increased from 7 to 16% when the Fe nanoparticles concentration increased from 0 to 1.5 g/L_R (the working volume in the bioreactor). Therefore, the CH₄ yield significantly promoted from 0.105 to 0.186 L/L_R. However, when the Fe nanoparticles concentration was further increased to 2 g/L_R, methanogenesis was inhibited due to toxic effects. The electron transfer constant k_app of anaerobic sludge increased by 32.8-fold to 5.77 × 10^-2 s^-1 when the Fe nanoparticles concentration increased from 0 to 1.5 g/L_R, which significantly promoted carbon conversion efficiency from 52.9 to 92.9% and energy conversion efficiency from 46.3 to 76.9%.