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Behavioral and Biochemical Effects of Mukia madrespatana Following Single Immobilization Stress on Rats
Noreen Samad1, Amna Ali1, Farzana Yasmin2,3
1Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
Background And Objectives:
Elevated oxidative stress has been shown to play an important role in the diagnosis and prognosis of stress and memory-related complications. Mukia madrespatana (M. madrespatana) has been reported to have various biological and antioxidant properties. We intended to evaluate the effect of M. madrespatana peel on single immobilization stress-induced behavioral deficits and memory changes in rats. Materials and Methods: M. madrespatana peel (2000 mg/kg/day, orally) was administered to control and immobilize stressed animals for 4 weeks. Anxiolytic, antidepressant, and memory-enhancing effects of M. madrespatana were observed in both unstressed and stressed animals. Results: Lipid peroxidation was decreased while antioxidant enzymes were increased in both unstressed and stressed animals. Acetylcholine level was increased while acetylcholinesterase activity was decreased in both M. madrespatana treated unstressed and stressed rats. There was also an improvement in memory function. Serotonin neurotransmission was also regulated in M. madrespatana treated rats following immobilization stress with anxiolytic and anti-depressive effects. Conclusion: Based on the current study, it is suggested that M. madrespatana has strong antioxidant properties and may be beneficial as dietary supplementation in stress and memory-related conditions.
Insights
Mukia madrespatana peel extract demonstrated significant antioxidant and memory-enhancing effects in rats. This natural compound may help mitigate stress-induced behavioral deficits and improve cognitive function.
Area of Science:
- Neuroscience
- Pharmacology
- Oxidative Stress Research
Background:
- Elevated oxidative stress is linked to stress and memory complications.
- Mukia madrespatana (M. madrespatana) exhibits known antioxidant properties.
- The role of M. madrespatana in stress and memory requires further investigation.
Purpose of the Study:
- To evaluate the impact of M. madrespatana peel on stress-induced behavioral and memory changes in rats.
- To assess the antioxidant effects of M. madrespatana in a stress model.
Main Methods:
- Rats were administered M. madrespatana peel (2000 mg/kg/day) orally for 4 weeks.
- Behavioral tests assessed anxiolytic, antidepressant, and memory-enhancing effects.
- Biochemical assays measured oxidative stress markers and neurotransmitter levels.
Main Results:
- M. madrespatana decreased lipid peroxidation and increased antioxidant enzymes.
- Acetylcholine levels rose, and acetylcholinesterase activity decreased.
- Improved memory function and regulated serotonin neurotransmission were observed, with anxiolytic and anti-depressive effects.
Conclusions:
- M. madrespatana possesses strong antioxidant properties.
- The extract demonstrated beneficial effects on stress-induced behavioral deficits and memory impairment.
- M. madrespatana shows potential as a dietary supplement for stress and memory-related conditions.
