Related Experiment Video

Updated: Dec 14, 2025

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.7K

TAF15 contributes to the radiation-inducible stress response in cancer

Abhay Kumar Singh1, Vaishali Kapoor1, Dinesh Thotala1,2

  • 1Department of Radiation Oncology, Washington University in St. Louis, St. Louis, Missouri, USA.

Oncotarget
|July 18, 2020
PubMed

Insights

TAF15 is a radiation-inducible protein that worsens survival in non-small cell lung cancer (NSCLC). Silencing TAF15 or targeting it with an antibody enhances radiation therapy effectiveness, offering new treatment strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy

Background:

  • Radiation resistance is a major challenge in non-small cell lung cancer (NSCLC) treatment.
  • Novel molecular targets are needed for combination therapies.
  • TAF15 was identified as a radiation-inducible protein.

Purpose of the Study:

  • To investigate the role of TAF15 in NSCLC radiation resistance.
  • To evaluate TAF15 as a therapeutic target for NSCLC.

Main Methods:

  • Phage-displayed peptide libraries to identify radiation-inducible targets.
  • Genetic silencing of TAF15 in NSCLC cells.
  • In vitro and in vivo studies of TAF15 expression and function.
  • Antibody-based targeting of surface-expressed TAF15.

Main Results:

  • TAF15 overexpression correlates with poorer survival in NSCLC patients.
  • Radiation induces TAF15 expression on the cell surface.
  • TAF15 depletion causes cell cycle arrest, apoptosis, and reduced proliferation.
  • Combined radiation and TAF15 knockdown significantly reduce cancer cell survival.
  • Anti-TAF15 antibody treatment combined with radiation decreases cancer cell survival.

Conclusions:

  • TAF15 is a radiation-inducible target that promotes NSCLC cell viability.
  • Surface-expressed TAF15 is accessible to antibody-based therapies.
  • Targeting TAF15 offers a promising strategy to overcome radiation resistance in NSCLC.
Keywords:
TAF15antibodylung cancerradiation inducible

More Related Videos

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

905
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells05:18

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Published on: June 9, 2020

11.6K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.7K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.6K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.9K