Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Molecular Biology

Background:

• Developing stimuli-responsive drug delivery systems is crucial for targeted therapies.
• Nanovesicles offer potential for efficient cellular delivery and controlled release.
• Photo-responsive materials enable spatiotemporal control over biological processes.

Purpose of the Study:

• To fabricate a novel photo-responsive nanovesicle with switchable stability.
• To investigate the UV-triggered gene knockdown capabilities of the nanovesicle.
• To explore the potential of polyion complex (PIC) formation in creating advanced nanostructures.

Main Methods:

• Fabrication of nanovesicles via polyion complex (PIC) formation between PEG-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs).
• Utilizing ultraviolet (UV) light to induce reversible crosslinking in the nanovesicle membrane.
• Assessing cellular internalization and gene knockdown efficiency in cultured cells.

Main Results:

• Successfully synthesized a photo-responsive nanovesicle with tunable stability.
• Demonstrated UV-triggered reversible crosslinking of PROs and ASOs within the nanovesicle membrane.
• Achieved efficient cellular uptake and significant UV-induced gene knockdown.

Conclusions:

• The developed photo-responsive nanovesicle exhibits switchable stability controlled by UV light.
• This nanovesicle platform shows promise for targeted gene therapy applications.
• The PIC strategy is effective for creating advanced, light-responsive nanostructures for biomedical use.