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A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Published on: August 21, 2018
Single nucleotide variant discrimination by toehold exchange spherical nucleic acids modulated on hierarchical
Min Zhu1, Yanyan Yu, Junfeng Zhu
1School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China. yuyanyan@ntu.edu.cn.
Abstract:
Toehold exchange spherical nucleic acids (TESNA) modulated on molybdenum disulfide (MoS2) acanthospheres were proposed for the discrimination of single nucleotide variants (SNVs). We rationally optimized the probe by making a trade-off between the discrimination factor (DF) and hybridization ratio (χ). The TESNA platform can distinguish SNVs with significantly improved sensitivity and specificity.
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