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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Tagging and Fusion Proteins01:24

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Related Experiment Video

Updated: Dec 14, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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Chemical Tools for Endogenous Protein Labeling and Profiling.

Kazuki Shiraiwa1, Rong Cheng1, Hiroshi Nonaka2

  • 1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.

Cell Chemical Biology
|July 18, 2020
PubMed
Summary

Analyzing proteins within live cells requires precise labeling to avoid artifacts. This review explores chemical biology techniques for selective protein labeling and modification under native conditions, offering alternatives to genetic methods.

Keywords:
activity-based proteome labelingchemical labeling toolsconditional proteomicsdesign guidelineendogenous proteinproximity-dependent proteome labeling

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Last Updated: Dec 14, 2025

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Understanding protein structure and function requires analysis under biological conditions.
  • Selective labeling of proteins of interest (POI) is crucial in complex cellular environments.
  • Genetic manipulation for protein labeling can lead to overexpression and cellular artifacts.

Purpose of the Study:

  • To review chemical biology approaches for analyzing endogenous proteins under native conditions.
  • To summarize techniques for protein-selective chemical labeling.
  • To discuss proteome-directed chemical modification strategies.

Main Methods:

  • Review of literature on chemical biology techniques for protein analysis.
  • Focus on methods enabling selective labeling of proteins of interest (POI).
  • Discussion of proteome-directed chemical modification strategies.

Main Results:

  • Two major chemical approaches for analyzing endogenous proteins under native conditions have emerged.
  • Protein-selective chemical labeling allows precise POI analysis.
  • Proteome-directed chemical modification offers alternative strategies.

Conclusions:

  • Chemical biology provides powerful tools for studying proteins in their native cellular context.
  • These methods overcome limitations associated with genetic manipulation in protein analysis.
  • Selective chemical labeling and modification are key for accurate protein studies.