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Updated: Dec 14, 2025

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

A solid-phase extraction method for estrogenic disrupting compounds based on the estrogen response element.

Jinghua Zhang¹, Liguo Zang¹, Tian Wang¹

¹Shandong Provincial Key Laboratory of Animal Resistance Biology, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China.

|July 18, 2020

Summary

A new method uses estrogen receptors to detect estrogenic disrupting compounds (EDCs) like BPA in milk. This sensitive technique ensures milk safety by identifying EDCs below regulatory limits.

Keywords:

Estrogen receptor Estrogen response element Estrogenic disrupting compounds High-performance liquid chromatography Solid-phase extraction

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Area of Science:

Environmental Chemistry
Analytical Chemistry
Food Safety

Background:

Estrogenic disrupting compounds (EDCs) pose risks to human health and ecosystems.
Accurate detection of EDCs in food matrices like milk is crucial for public health.
Existing methods may lack the sensitivity or specificity required for complex samples.

Purpose of the Study:

To establish a sensitive solid-phase extraction (SPE) method for enriching and purifying EDCs.
To develop a reliable analytical approach for quantifying EDCs in milk products.
To assess the levels of specific EDCs in commercially available milk samples.

Main Methods:

Solid-phase extraction (SPE) utilizing the estrogen receptor for molecular recognition of EDCs.
Estrogen response element incorporated to maintain estrogen receptor activity.
High-performance liquid chromatography (HPLC) for the quantification of detected EDCs.

Main Results:

Successful enrichment and purification of EDCs from a liquid milk matrix.
High recoveries (84.1% to 113.6%) for target EDCs including bisphenol A (BPA), 17β-estradiol, and diethylstilbestrol.
Low limits of detection (1 × 10⁻⁶ mg·mL⁻¹) and quantification (5 × 10⁻⁶ mg·mL⁻¹).
Bisphenol A (BPA) was detected in one liquid milk sample, below regulatory limits.

Conclusions:

The developed SPE-HPLC method is effective for detecting and quantifying EDCs in milk.
The method demonstrates high sensitivity and specificity, suitable for food safety analysis.
Market milk samples analyzed showed minimal contamination with EDCs, with detected levels below safety thresholds.