Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DM-CFO: A Diffusion Model for Compositional 3D Tooth Generation With Collision-Free Optimization.

IEEE transactions on visualization and computer graphics·2026

Same author

Roadmap on computational methods in optical imaging and holography [invited].

Applied physics. B, Lasers and optics·2024

Same author

Miniature color camera via flat hybrid meta-optics.

Science advances·2023

Same author

Hybrid diffractive optics design via hardware-in-the-loop methodology for achromatic extended-depth-of-field imaging.

Optics express·2022

Same author

Phase Retardation Analysis in a Rotated Plane-Parallel Plate for Phase-Shifting Digital Holography.

Journal of imaging·2022

Same author

Power-balanced hybrid optics boosted design for achromatic extended depth-of-field imaging via optimized mixed OTF.

Applied optics·2021

Search research articles

Related Experiment Video

Updated: Dec 14, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Lensless hyperspectral phase imaging in a self-reference setup based on Fourier transform spectroscopy and noise

Igor Shevkunov, Vladimir Katkovnik, Karen Egiazarian

|July 19, 2020

Summary

This study introduces a new phase retrieval algorithm for hyperspectral imaging, improving phase reconstruction from noisy data. The method effectively filters noise, enabling precise imaging and depth profiling of transparent objects.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Dec 14, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

Optical Physics
Image Processing
Spectroscopy

Background:

Hyperspectral imaging requires accurate phase information for detailed analysis.
Existing phase retrieval methods struggle with noisy intensity observations.
Fellgett's disadvantage in Fourier transform spectroscopy amplifies noise, complicating phase reconstruction.

Purpose of the Study:

To develop a novel phase retrieval algorithm for broadband hyperspectral phase imaging.
To overcome limitations of existing methods in handling noisy data.
To reconstruct both the phase and depth profile of objects.

Main Methods:

Utilizing Fourier transform spectroscopy in a self-referencing optical setup.
Implementing a sparse wavefront noise filtering technique within the algorithm.
Validating the algorithm through simulations and physical experiments with transparent objects.

Main Results:

The algorithm successfully reconstructs the phase distribution of investigated objects.
Noise amplification, a known issue (Fellgett's disadvantage), is significantly mitigated.
Precise phase imaging and accurate object depth (profile) reconstruction were demonstrated.

Conclusions:

The proposed algorithm offers a robust solution for hyperspectral phase imaging from noisy data.
It enhances phase reconstruction accuracy and enables detailed object profiling.
The method shows promise for applications requiring high-resolution imaging of transparent materials.