Related Experiment Video
Updated: Dec 14, 2025
06:12
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
1.9K
High-throughput imaging surface plasmon resonance biosensing based on ultrafast two-point spectral-dip tracking
Optics Express
|July 19, 2020
Summary
We developed an ultrafast wavelength interrogation surface plasmon resonance imaging (λSPRi) biosensor, achieving the fastest resonance wavelength measurement speed of 0.25s. This breakthrough enables rapid, high-throughput, label-free detection of biomolecular interactions.
Area of Science:
- Biophotonics and Biosensing
- Surface Plasmon Resonance Imaging
- Biomolecular Interaction Analysis
Background:
- Wavelength interrogation surface plasmon resonance imaging (λSPRi) offers potential for 2D sensor array detection.
- Current λSPRi systems face limitations in imaging rate, hindering real-time biomolecular binding detection.
Purpose of the Study:
- To demonstrate an ultrafast λSPRi biosensor system for real-time biomolecular detection.
- To overcome the speed limitations of conventional λSPRi systems.
Main Methods:
- Development of an ultrafast λSPRi biosensor system.
- Implementation of a two-point tracking algorithm to drive a liquid crystal tunable filter (LCTF).
- Fast-tracking of resonance wavelength shifts caused by molecular binding events.
Main Results:
- Achieved a resonance wavelength measurement time of 0.25s, the fastest reported for λSPRi.
- Demonstrated high sensitivity (2.4 × 10-6 RIU) and dynamic range (4.6 × 10-2 RIU).
- Successfully performed fast, high-throughput detection of biomolecular interactions.
Conclusions:
- The developed ultrafast λSPRi system significantly enhances real-time biomolecular detection capabilities.
- This technology is highly suitable for high-throughput and label-free biosensing applications.
- The two-point tracking algorithm is key to achieving unprecedented measurement speeds.
