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Quantitative membrane loading of polymer vesicles.

P Peter Ghoroghchian¹, John J Lin², Aaron K Brannan³

¹School of Engineering and Applied Science, and Institute for Medicine and Engineering, University of Pennsylvania, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, PA 19104-6323, USA. hammer@seas.upenn.edu and Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104-6323, USA. therien@sas.upenn.edu.

|July 19, 2020

Summary

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This summary is machine-generated.

Polymer vesicles (polymersomes) can stably incorporate large hydrophobic molecules within their membranes. This method prevents aggregation and shields molecules, creating advanced multi-functional materials.

Area of Science:

Materials Science
Supramolecular Chemistry
Nanotechnology

Background:

Polymer vesicles (polymersomes) are synthetic mimics of biological membranes.
Incorporating hydrophobic molecules into polymersomes is challenging.
Existing methods often compromise vesicle stability.

Purpose of the Study:

To investigate the stable incorporation of large hydrophobic molecules into polymersome membranes.
To explore the use of multi-porphyrin-based, fluorophores (PBFs) for this purpose.
To assess the impact of hydrophobic molecule loading on polymersome stability and functionality.

Main Methods:

Self-assembly of polymersomes via hydration of polymer and PBF thin films.
Spectroscopic analysis (absorption and emission) to verify PBF loading.

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Cryogenic transmission electron microscopy (cryo-TEM) and micropipet aspiration to assess stability.

Main Results:

Polymersomes successfully incorporated hydrophobic PBFs up to 10 mol/wt% concentration.
Hydrophobic molecules ranged from 1.4-5.4 nm in length and 0.7-5.4 kg/mol.
Vesicle thermodynamic and mechanical stabilities were not significantly compromised.
Incorporated hydrophobic molecules were prevented from aggregation and shielded from the environment.

Conclusions:

Polymersomes offer a robust platform for incorporating large hydrophobic molecules.
This approach enables high concentration and protection of hydrophobic agents.
A generalized method for creating complex, multi-functional materials via self-assembly is presented.