Related Experiment Video
Updated: Dec 14, 2025
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
Lin Yuan1, Yingyi Liu1, Yana Chen1
1China Animal Disease Control Center, OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, No. 17 Tiangui Street, Biomedical Base, Daxing District, Beijing, 102618, China.
Background:
Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays.
Result:
In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region within Capsid genome of PCV3 was selected for the design of primer pairs and probes. After optimizing, a primer pair and probe was screened, providing high sensitivity (10 copies/μL) and specificity (no cross reaction with other porcine viruses or common bacterium). In addition, this method was applied in the detection of 110 clinical samples, and the performance was compared with other previously reported PCR and real-time PCR methods. This method provided higher detection rate.
Conclusion:
A real-time fluorescence PCR assay has been developed for the detection of PCV3, with high sensitivity and specificity, exhibiting good performance in detecting clinical samples.
Insights
A new real-time PCR assay effectively detects Porcine circovirus type 3 (PCV3) in pigs. This highly sensitive and specific method offers improved detection rates for this emerging swine virus.
Area of Science:
- Veterinary Virology
- Molecular Diagnostics
- Swine Disease Surveillance
Background:
- Porcine circovirus type 3 (PCV3) is an emerging global swine pathogen.
- PCV3's conserved DNA sequence is suitable for molecular diagnostic assays.
Purpose of the Study:
- To develop a sensitive and specific real-time fluorescence PCR assay for PCV3 detection.
- To evaluate the assay's performance on clinical samples.
Main Methods:
- Designed primers and probes targeting the conserved Capsid gene of PCV3.
- Optimized the real-time fluorescence PCR assay.
- Validated assay sensitivity and specificity.
- Applied the assay to 110 clinical swine samples.
Main Results:
- The developed assay demonstrated high sensitivity (10 copies/μL).
- The assay showed high specificity, with no cross-reactivity with other swine viruses or bacteria.
- The method achieved a higher detection rate compared to previously reported PCR techniques in clinical samples.
Conclusions:
- A novel real-time fluorescence PCR assay for PCV3 detection has been successfully developed.
- The assay is highly sensitive and specific, proving effective for clinical sample analysis.
- This method offers an improved diagnostic tool for PCV3 surveillance in swine populations.
