Related Experiment Video

Updated: Dec 14, 2025

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
09:31

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Published on: October 16, 2016

9.8K

Dopamine induces in vitro migration of synovial fibroblast from patients with rheumatoid arthritis

Lina van Nie1, Laura Salinas-Tejedor2, Nicole Dychus2

  • 1Department of Internal Medicine and Rheumatology, Justus-Liebig-University Gießen, Campus Kerckhoff-Klinik, Benekestr. 2-8, 61231, Bad Nauheim, Germany.

Scientific Reports
|July 19, 2020
PubMed

Insights

Dopamine signaling influences the migration of synovial fibroblasts in rheumatoid arthritis (RA) and osteoarthritis (OA). Targeting dopamine receptors may offer a new therapeutic strategy for preventing joint damage in arthritis.

Area of Science:

  • Rheumatology
  • Cell Biology
  • Pharmacology

Background:

  • Synovial fibroblast (SF) migration into cartilage is a key target for rheumatoid arthritis (RA) therapy.
  • Dopamine receptors (DR) are expressed by SF in RA and osteoarthritis (OA).

Purpose of the Study:

  • To investigate the effect of dopamine on the mobility of fibroblasts from patients with chronic arthritides.
  • To explore the potential of the dopaminergic pathway as a therapeutic target in RA.

Main Methods:

  • Obtained synovial tissue and fibroblasts from RA and OA patients.
  • Performed immunohistochemistry for DR subtypes, migration and motility assays under DR stimulation.
  • Evaluated cytokines via ELISA, DR expression by flow cytometry, and DR activation using xCELLigence real-time analysis.

Main Results:

  • All DR subtypes were expressed in the RA invasion zone.
  • Dopamine receptor stimulation increased migration and motility of SF in patients ≤75 years old.
  • Older RA patients (>75 years) showed lower D1, D2, and D4 DR expression, with unchanged DR activation.

Conclusions:

  • Dopamine influences fibroblast migration in arthritis patients.
  • The synovial dopaminergic pathway presents a potential therapeutic target to mitigate joint damage in RA.

More Related Videos

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

830
Preliminary Study on Acupuncture Combined with Grain-sized Moxibustion for Treating Rheumatoid Arthritis with Finger Joint Pain04:50

Preliminary Study on Acupuncture Combined with Grain-sized Moxibustion for Treating Rheumatoid Arthritis with Finger Joint Pain

Published on: May 16, 2025

665

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.9K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.0K
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.1K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.1K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.2K