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Proteomic profiling of developing wheat heads under water-stress
Shahidul Islam1, Penghao Wang2, Delphine Vincent3
1Australia China Centre for Wheat Improvement, College of Science Health Engineering and Education, Murdoch University, 90 South Street, Murdoch, WA, 6150, Australia.
Functional & Integrative Genomics
|July 19, 2020
Summary
Water-stress impacts wheat head development by altering protein expression, affecting seed set. This study used isobaric tags for relative and absolute quantification (iTRAQ) to identify key proteins involved in stress response and developmental changes.
Area of Science:
- Plant Science
- Proteomics
- Agricultural Science
Background:
- Water-stress during reproductive stages significantly impacts crop yield.
- Understanding proteome dynamics in developing wheat heads under stress is crucial for breeding resilient varieties.
Purpose of the Study:
- To characterize proteome alterations in developing wheat heads under water-stress.
- To identify proteins responsive to water-stress across different head developmental stages.
- To correlate proteomic changes with seed set phenotypes in response to water-stress.
Main Methods:
- Utilized isobaric tags for relative and absolute quantification (iTRAQ) for proteomic analysis.
- Analyzed 120 samples from two wheat doubled haploid (DH) lines under water-stressed and control conditions.
- Collected samples across five head developmental stages, from early booting to post-anthesis.
Main Results:
- Identified 132 proteins with significant water-stress-responsive changes, including down-regulation of mitochondrial Rho GTPase and FtsZ, and up-regulation of IMP dehydrogenase and glycerophosphodiester phosphodiesterase.
- Differentiated proteomes between pre-head emergence (Pre-FHE), full head emergence (FHE), and post-FHE stages.
- Observed reduced seed set (27% and 6%) in DH lines and identified 7 water-stress-influenced proteins correlating with this phenotype, such as a subtilisin-like protease.
Conclusions:
- Water-stress significantly alters the proteome of developing wheat heads, impacting fundamental biological processes.
- Specific proteins are identified as key players in wheat's response to reproductive stage water-stress.
- Proteomic insights can inform breeding strategies for enhanced drought tolerance and yield stability in wheat.
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