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Related Experiment Video

Updated: Dec 14, 2025

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging.

Lalhaba Oinam^1,2, Gopakumar Changarathil^1,3, Erna Raja^1,4

¹Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan.

|July 19, 2020

Summary

Skin aging involves epidermal stem cell changes. Researchers found distinct glycan profiles in aged epidermal stem cells, suggesting these sugar patterns could serve as biomarkers for skin aging.

Keywords:

epidermal stem cells glycosylation lectin microarray mannose sialylation skin aging stem cell aging

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Last Updated: Dec 14, 2025

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

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Published on: December 17, 2012

Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Published on: May 22, 2016

Area of Science:

Dermatology
Stem Cell Biology
Glycobiology

Background:

Epidermal aging leads to reduced barrier function, slower wound healing, hair loss, and increased cancer risk.
Age-related changes in epidermal stem cells and their microenvironment are implicated in skin aging.
Current methods lack biochemical tools to assess epidermal stem cell aging.

Purpose of the Study:

To investigate changes in glycans of epidermal stem cells as potential biomarkers of aging.
To explore the role of glycosylation in epidermal stem cell function during aging.

Main Methods:

Comprehensive glycan profiling of epidermal stem cells from young and old mice using lectin microarray.
Analysis of gene expression for sialyltransferases (St3gal2, St6gal1) and mannosidase (Man1a).
Functional assessment of epidermal stem cell regenerative ability in culture after glycan modification.

Main Results:

Significant differences in glycan profiles were observed between young and old epidermal stem cells.
Decreased binding of mannose-specific lectin (rHeltuba) and increased binding of α2-3Sia-specific lectin (rGal8N) in aged cells.
Upregulation of St3gal2, St6gal1, and Man1a genes in aged epidermal stem cells, correlating with impaired regenerative capacity.

Conclusions:

Age-related alterations in cellular glycosylation patterns affect epidermal stem cell function.
Detected glycan modifications can serve as molecular markers for skin aging.
Further research into these glycan changes will enhance understanding of the skin aging process.