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Updated: Dec 14, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Textured nanofibrils drive microglial phenotype.

Qin Song¹, Simone Pifferi², Lin Shi³

¹International School for Advanced Studies (SISSA), Trieste, 34136, Italy; School of Pharmaceutical Engineering, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, PR China; Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang, 315201, PR China.

|July 19, 2020

Summary

This summary is machine-generated.

Modified bacterial cellulose (mBC) nanofibril substrates promote microglia with in vivo-like processes and altered ion channel activity. This creates a novel microglia-material interface for anti-neuroinflammatory drug screening.

Keywords:

Aging-like phenotype Microglia Nanofibril Potassium channels Ramification

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Published on: October 27, 2019

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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

Area of Science:

Neuroscience
Biomaterials Science
Cell Biology

Background:

Microglia are immune cells in the central nervous system, crucial for brain health and disease.
Microglial behavior is highly influenced by their surrounding microenvironment.
Understanding microglial responses to biomaterials is key for developing neural interfaces and therapies.

Purpose of the Study:

To investigate how modified bacterial cellulose (mBC) nanofibril substrates affect microglial properties.
To explore the potential of mBC as a platform for studying microglial behavior and for drug screening.

Main Methods:

Immunofluorescence microscopy
Live-cell imaging
Electrophysiological recordings (patch-clamp)
RNA sequencing (transcriptome analysis)

Main Results:

mBC substrates induced ramified microglia with dynamic processes, mimicking in vivo morphology.
Microglia on mBC showed a more negative resting membrane potential and increased inward rectifier K+ currents due to Kir2.1 channel upregulation.
Transcriptome analysis revealed altered gene expression related to immune response, cell adhesion, and motility.
Arp2/3 complex activation and integrin signaling were identified as modulators of microglial morphology and motility.

Conclusions:

mBC nanofibril substrates effectively modulate microglial phenotype, promoting a more physiological state.
The mBC-microglia interface shows promise for developing advanced tools for anti-neuroinflammatory drug discovery and screening.