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Updated: Dec 14, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
Variability and additivity of read counts for aSTRs in NGS DNA profiles
Kevin Cheng1, Jessica Skillman2, Stephanie Hickey2
1Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland, 1142 New Zealand; Department of Statistics, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Abstract:
There has been an increase in the number of laboratories and researchers adopting new sequencing technologies, known as next-generation sequencing (NGS). An understanding of the behaviour of NGS DNA profiles is needed to enable for the development of probabilistic genotyping methods for the interpretation of such profiles. In this work, we investigate NGS analyte signal variation, specifically heterozygous balance and stutter variability from profiles generated using the ForenSeq™ DNA Signature Prep Kit, DNA Primer Mix B. We also investigate additivity of analyte signals in NGS profiles for overlapping allelic and stutter signals originating from the same or different contributors. We describe models that can be used to inform a continuous method for the interpretation of DNA profiling data.
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