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Updated: Dec 14, 2025

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Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase

Mônica Ghislaine Oliveira Alves1, Natália da Silva Miguel2, Camila Cristina Panisello Ferreira3

  • 1School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil; School of Dentistry, Brazcubas Educação, Mogi das Cruzes, Brazil; School of Medicine, Anhembi Morumbi University, São José dos Campos, Brazil.

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
|July 20, 2020
PubMed
Abstract

Insights

DNA repair gene expression, including MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2, was significantly reduced in oral squamous cell carcinoma (OSCC) patients compared to controls.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent cancer with complex genetic underpinnings.
  • DNA repair mechanisms are crucial for maintaining genomic stability and preventing cancer development.

Purpose of the Study:

  • To investigate the expression levels of key DNA repair genes in OSCC tissues.
  • To compare gene expression between OSCC patients and a healthy control group.

Main Methods:

  • Gene expression analysis using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
  • Evaluation of MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 gene expression.
  • Statistical comparison using Mann-Whitney test with Bonferroni correction and correlation analysis.

Main Results:

  • Significant downregulation of MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was observed in OSCC tissues.
  • Specific P-values indicate high statistical significance for the observed downregulation across most genes.
  • A negative correlation was found between age and PMS2 gene expression.

Conclusions:

  • The study demonstrates reduced expression of critical DNA repair genes in OSCC.
  • These findings suggest a potential role for DNA repair gene dysfunction in OSCC pathogenesis.
  • Further research could explore therapeutic strategies targeting DNA repair pathways in OSCC.

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