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Resveratrol Suppresses Human Nasopharyngeal Carcinoma Cell Growth Via Inhibiting Differentiation Antagonizing
Jiafeng Zhang1, Xiaojie Jin1, Chuan Zhou1
1Department of Otorhinolaryngology, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China (mainland).
Abstract:
BACKGROUND Although resveratrol has been found to show anti-cancer effects and potential chemotherapeutic activities in several cancers, the role and molecular mechanisms of resveratrol in nasopharyngeal carcinoma (NPC) remains poorly understood. This study aimed to investigate the effect of resveratrol in NPC progression and its molecular mechanism. MATERIAL AND METHODS Quantitative real-time polymerase chain reaction and western blotting were used to detect the expression of DANCR and PTEN. MTT assay and EdU assay were performed to detect the cell proliferation in NPC cells with different treatment. The effect of resveratrol on cell migration was explored by Transwell migration assay. RNA immunoprecipitation assay and chromatin immunoprecipitation assay were performed to test the interaction between DANCR, EZH2, and PTEN. A mouse xenograft model of NPC cell was established, and immunohistochemistry assay was performed to detect the PTEN expression. RESULTS Resveratrol treatment inhibited NPC cell growth and migration in a dose-dependent manner. Additionally, resveratrol downregulated the expression of DANCR and DANCR overexpressing abrogated the inhibition effect of resveratrol on NPC cell migration. Mechanistically, DANCR could bind to EZH2 and downregulated PTEN expression through mediating the binding of EZH2 on PTEN promoter. Furthermore, rescue experiments suggested resveratrol inhibited NPC cell growth and migration by the DANCR/PTEN pathway. Resveratrol significantly decreased the tumor volume and tumor weight and increased the expression of PTEN. CONCLUSIONS Resveratrol increased PTEN expression and suppressed NPC cell growth and migration through downregulation of DANCR.
Insights
Resveratrol, a natural compound, inhibits nasopharyngeal carcinoma (NPC) progression by decreasing DANCR expression, which upregulates PTEN. This study clarifies resveratrol's anti-cancer mechanism in NPC.
Area of Science:
- Oncology
- Molecular Biology
- Natural Product Chemistry
Background:
- Resveratrol exhibits anti-cancer properties, but its role in nasopharyngeal carcinoma (NPC) is unclear.
- Understanding resveratrol's molecular mechanisms in NPC is crucial for potential therapeutic development.
Purpose of the Study:
- To investigate the effects of resveratrol on NPC progression.
- To elucidate the molecular mechanisms underlying resveratrol's action in NPC.
Main Methods:
- Quantitative real-time PCR and Western blotting for DANCR and PTEN expression.
- Cell proliferation (MTT, EdU) and migration (Transwell) assays.
- RNA immunoprecipitation, chromatin immunoprecipitation, and mouse xenograft models.
Main Results:
- Resveratrol inhibited NPC cell growth and migration dose-dependently.
- Resveratrol downregulated DANCR expression; DANCR overexpression counteracted resveratrol's effects.
- Resveratrol suppressed NPC progression via the DANCR/PTEN pathway, increasing PTEN expression.
Conclusions:
- Resveratrol suppresses NPC growth and migration by downregulating DANCR.
- This mechanism involves increasing PTEN expression, offering a potential therapeutic strategy for NPC.
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