A low voltage activated Ca²⁺ current found in a subset of human ventricular myocytes

Low voltage activated (I_Ca-LVA) calcium currents including Cav1.3 and T-type calcium current (I_Ca-T) have not been reported in adult human left ventricular myocytes (HLVMs). We tried to examine their existence and possible correlation with etiology and patient characteristics in a big number of human LVMs isolated from explanted terminally failing (F) hearts, failing hearts with left ventricular assist device (F-LVAD) and nonfailing (NF) human hearts. LVA (I_Ca-LVA) was determined by subtracting L-type Ca²⁺ current (I_Ca-L) recorded with the holding potential of -50 mV from total Ca²⁺ current recorded with the holding potential of -90 mV or -70 mV. I_{Ca- LVA} was further tested with its sensitivity to 100 µM CdCl₂ and tetrodotoxin. Three HLVMs (3 of 137 FHLVMs) from 2 (N = 30 hearts) failing human hearts, of which one was idiopathic and the other was due to primary pulmonary hypertension, were found with I_Ca-LVA. I_Ca-LVA in one FHLVM was not sensitive to 100 µM CdCl₂ while I_Ca-LVA in another two FHLVMs was not sensitive to tetrodotoxin. It peaked at the voltage of -40~-20 mV and had a time-dependent decay faster than I_Ca-L but slower than sodium current (I_Na). I_Ca-LVA was not found in any HLVMs from NF (75 HLVMs from 17 hearts) or F-LVAD hearts (82 HLVMs from 18 hearts) but a statistically significant correlation could not be established. In conclusion, I_Ca-LVA was detected in some HLVMs of a small portion of human hearts that happened to be nonischemic failing hearts.