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Updated: Dec 14, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.1K

MCM3AP-AS1/miR-876-5p/WNT5A axis regulates the proliferation of prostate cancer cells

Jie Wu1, Yalin Lv2, Yujun Li1

  • 1Department of Pathology, The Affiliated Hospital of Qingdao University, Jiangsu Road, South District, Qingdao, 266003 Shandong China.

Cancer Cell International
|July 21, 2020
PubMed
Abstract

Insights

Long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) is upregulated in prostate cancer, inhibiting proliferation and increasing apoptosis. It acts as a ceRNA, targeting miR-876-5p to regulate WNT5A expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) is recognized as oncogenic in various cancers.
  • Its specific role and expression patterns in prostate cancer remain underexplored.

Purpose of the Study:

  • To investigate the expression and functional role of MCM3AP-AS1 in prostate cancer.
  • To elucidate the molecular mechanism involving MCM3AP-AS1, miR-876-5p, and WNT5A in prostate cancer progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess MCM3AP-AS1, miR-876-5p, and WNT5A expression in prostate cancer tissues.
  • In vitro functional assays including cell proliferation (CCK-8, EdU) and apoptosis (flow cytometry) after MCM3AP-AS1 knockdown.
  • Luciferase reporter assays to confirm regulatory interactions between MCM3AP-AS1, miR-876-5p, and WNT5A.

Main Results:

  • MCM3AP-AS1 was significantly upregulated in prostate cancer tissues, correlating positively with WNT5A and negatively with miR-876-5p.
  • Knockdown of MCM3AP-AS1 inhibited cancer cell proliferation and induced apoptosis.
  • MCM3AP-AS1 functions as a competing endogenous RNA (ceRNA) for miR-876-5p, thereby upregulating WNT5A.

Conclusions:

  • MCM3AP-AS1 is aberrantly overexpressed in prostate cancer and influences cancer cell proliferation and apoptosis.
  • MCM3AP-AS1 may serve as a potential ceRNA, modulating WNT5A expression via the miR-876-5p pathway in prostate cancer.
Keywords:
MCM3AP-AS1ProliferationProstate cancerWNT5AmiR-876-5p

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