Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.8K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.8K
Histone Modification02:32

Histone Modification

15.5K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.5K
Histone Modification02:32

Histone Modification

4.1K
4.1K
Master Transcription Regulators02:23

Master Transcription Regulators

7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.1K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

5.6K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors of early cancer burden in <i>CDH1</i> pathogenic variant carriers: a UK single-centre cohort study.

EClinicalMedicine·2026
Same author

Forging New Pathways in Oncology: Strategic Insights from the 17th Annual Frontiers in Cancer Science Conference.

Cancer research·2026
Same author

PLA2 driven lipid signaling drives ARMS tumorigenic cell properties.

Cell communication and signaling : CCS·2025
Same author

Charting New Paths in Cancer Research: Insights from the Frontiers in Cancer Science Conference 2024.

Cancer research·2025
Same author

Clinical decision tree for optimizing endoscopic assessment of signet ring cell carcinoma in hereditary diffuse gastric cancer surveillance.

Endoscopy·2025
Same author

EHMT1 mediates cellular motility in embryonal rhabdomyosarcoma by activating SOX8 expression.

British journal of cancer·2025

Related Experiment Video

Updated: Dec 14, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.1K

Histone variants in skeletal myogenesis.

Nandini Karthik1, Reshma Taneja1

  • 1Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore , Singapore.

Epigenetics
|July 21, 2020
PubMed
Summary

Histone variants are crucial for muscle development by regulating gene expression. Understanding their roles and interactions is key to deciphering myogenesis and muscle diseases.

Area of Science:

  • Epigenetics and Molecular Biology
  • Cellular and Developmental Biology

Background:

  • Histone variants critically influence chromatin structure and gene transcription.
  • Skeletal myogenesis, regulated by MyoD, requires precise temporal control of gene expression.
  • Specific histone variants like H3.3, H2A.Z, macroH2A, and H1b are known to be important for muscle differentiation.

Purpose of the Study:

  • To review the roles of histone variants in modifying chromatin landscapes for proper muscle differentiation.
  • To highlight the importance of understanding the interplay between histone variants and their chaperones in myogenesis.

Main Methods:

  • This review synthesizes existing research on histone variants and myogenesis.
  • It focuses on the functional significance of histone variant substitutions in nucleosomes.
Keywords:
Skeletal myogenesisepigeneticshistone variantsmyopathies

More Related Videos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.4K
Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy
07:20

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy

Published on: October 18, 2024

846

Related Experiment Videos

Last Updated: Dec 14, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.1K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.4K
Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy
07:20

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy

Published on: October 18, 2024

846
  • The review examines the temporal regulation and cross-talk of histone variants and chaperones.

    • Main Results:

    • Histone variant substitutions profoundly alter nucleosomal architecture and chromatin landscape.
    • Timed substitution of histone variants ensures the sequential expression of myogenic genes.
    • Evidence supports the critical role of H3.3, H2A.Z, macroH2A, and H1b in skeletal myogenesis.

    Conclusions:

    • Histone variants are essential regulators of chromatin accessibility and gene transcription during muscle differentiation.
    • Further investigation into the relative importance of different histone variants and their chaperones is needed.
    • Understanding these epigenetic mechanisms is vital for comprehending myogenesis and associated myopathies.