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Updated: Dec 14, 2025
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
Gold nanoclusters/graphene quantum dots complex-based dual-emitting ratiometric fluorescence probe for the
Guo-Lin Hong1, Hao-Hua Deng2, Hai-Ling Zhao3
1Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
Abstract:
Herein, we report the design of a single-excitation/double-emission ratiometric fluorescence nanosensor for the determination of glucose. The sensing system combines glucose oxidation catalyzed by glucose oxidase, Fenton chemistry, Fe3+-sensitive fluorescent gold nanoclusters (AuNCs), and Fe3+-inert fluorescent graphene quantum dots (GQDs). We used orange-fluorescent AuNCs co-modified with bovine serum albumin and 3-mercaptopropionic acid as the indicator probe, and GQDs with the same excitation wavelength as the BSA/MPA-AuNCs, but with different emission wavelength, as the reference probe. The fluorescence intensity-ratio between 420 nm and 575 nm (F420/F575) was used to quantitatively determine glucose with a low detection limit of 0.18 μM, and the nanosensor was successfully used to detect glucose in human serum. This ratiometric fluorescence sensing system, based on AuNCs and GQDs, ensures sensitive and convenient determination of glucose, and has broad application prospects for biomedical-analysis applications.
