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Updated: Dec 14, 2025

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Achieving efficient nitrite accumulation in glycerol-driven partial denitrification system: Insights of influencing
Teng Zhang1, Jiashun Cao2, Yilei Zhang1
1Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.
Abstract:
Partial denitrification (PD), which could provide sufficient nitrite for subsequent anaerobic ammonium oxidation, is a novel strategy for mainstream nitrogen removal. In this study, the performance of using glycerol as electron donor for nitrite accumulation in PD process was evaluated. Results showed that a C/N of 4.5 was effective for nitrite production (average nitrite accumulation rate: 34.32 mg N h-1 gMLVSS-1; average nitrate-to-nitrite transformation ratio (NTR): 91.1%) with pH ranging from 6.0 to 9.0. Also, a stable nitrite accumulation was achieved in long-term operation with the average NTR of 80.1%. Mechanism investigation found that the denitrifying bacteria Saccharibacteria (77.9%) was enriched in glycerol-driven reactors. Moreover, the enzymatic activity as well as the encoding genes (i.e. narG, narH and napA) involved in nitrate reduction were much higher than that for nitrite reduction (i.e. nirK), and this disparity was responsible for the efficient nitrite accumulation in glycerol-driven PD system.
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