Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.0K
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.3K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.3K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

876
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
876
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.5K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.5K
Chirality in Nature02:30

Chirality in Nature

16.2K
Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.
16.2K
Chirality02:25

Chirality

28.6K
Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot.
Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...
28.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effective synchronization amid noise-induced chaos.

Physical review. E·2026
Same author

Transition to chaos with conical billiards.

Physical review. E·2025
Same author

Intrinsic Wrinkling of Free-Standing Polycrystalline Atomically Thin Films.

ACS nano·2025
Same author

Shear thickening in suspensions of particles with dynamic brush layers.

Soft matter·2024
Same author

Faceted wrinkling by contracting a curved boundary.

Soft matter·2024
Same author

Granular convergence as an iterated local map.

The European physical journal. E, Soft matter·2023

Related Experiment Video

Updated: Dec 14, 2025

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.4K

Chiral motion in colloidal electrophoresis.

Lara Braverman1, Aaron Mowitz1, Thomas A Witten1

  • 1Department of Physics and James Franck Institute, University of Chicago, Chicago, Illinois 60637, USA.

Physical Review. E
|July 22, 2020
PubMed
Summary

Predicting colloidal particle motion under electric fields is complex. This study presents a new method to calculate particle movement, revealing chiral twisting even in symmetric shapes.

Area of Science:

  • Colloid and Interface Science
  • Soft Matter Physics
  • Computational Electrodynamics

Background:

  • Asymmetrically charged, nonspherical colloidal particles exhibit complex translational and rotational motion when subjected to electric fields.
  • Predicting these motions is challenging due to the intricate interplay between electrostatic and hydrodynamic forces.

Purpose of the Study:

  • To develop a computational method for calculating the body tensors that govern the translational and rotational velocities of colloidal particles in response to external electric fields.
  • To validate this method against known electrophoretic mobilities for various particle shapes and charge distributions.

Main Methods:

  • Representing rigid, insulating bodies as an assembly of point sources for hydrodynamic drag and surface electric fields.

More Related Videos

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

12.4K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

34.8K

Related Experiment Videos

Last Updated: Dec 14, 2025

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.4K
Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
10:56

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

12.4K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

34.8K
  • Applying linear-response theory with an assumption of negligible electrostatic screening length.
  • Calculating body tensors that determine particle velocity vectors.

    • Main Results:

    • The developed method accurately predicts electrophoretic mobility for diverse particle shapes and charge configurations, with deviations within a few percent.
    • Demonstrated that even geometrically symmetric particles can exhibit significant chiral twisting motions under electric fields.
    • The calculational framework shows broad applicability to other active colloidal systems.

    Conclusions:

    • The presented method offers a robust approach to predict the complex electrokinetic behavior of colloidal particles.
    • This work provides new insights into chiral dynamics in colloidal systems and has implications for designing active micro- and nanodevices.