Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes01:24

Peroxisomes

19.5K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
19.5K
Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

4.2K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
4.2K
Autoxidation of Ethers to Peroxides and Hydroperoxides02:23

Autoxidation of Ethers to Peroxides and Hydroperoxides

9.1K
Ethers represent a class of chemical compounds that become more dangerous with prolonged storage because they tend to form explosive peroxides when standing in the air. Autoxidation is the spontaneous oxidation of a compound in air. In the presence of oxygen, ethers slowly oxidize to form hydroperoxides and dialkyl peroxides.
9.1K
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

93.5K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
93.5K
Pyruvate Oxidation01:15

Pyruvate Oxidation

167.7K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
167.7K
Radical Autoxidation01:20

Radical Autoxidation

2.8K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tomato cell cultures as innovative plant biostimulants: evaluation of its effect on pepper seeds germinated under salinity conditions.

Frontiers in plant science·2025
Same author

Higher Plant-Derived Biostimulants: Mechanisms of Action and Their Role in Mitigating Plant Abiotic Stress.

Antioxidants (Basel, Switzerland)·2024
Same author

Factors Affecting the Bioproduction of Resveratrol by Grapevine Cell Cultures under Elicitation.

Biomolecules·2023
Same author

Biotechnological Approach to Increase Oxyresveratrol Production in Mulberry In Vitro Plants under Elicitation.

Plants (Basel, Switzerland)·2023
Same author

Cyclodextrins Increase Triterpene Production in <i>Solanum lycopersicum</i> Cell Cultures by Activating Biosynthetic Genes.

Plants (Basel, Switzerland)·2022
Same author

Biotechnological production of β-carotene using plant in vitro cultures.

Planta·2022

Related Experiment Video

Updated: Dec 14, 2025

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

3.0K

Peroxidase: a multifunctional enzyme in grapevines.

Alfonso Ros Barceló1, Federico Pomar2, Matías López-Serrano2

  • 1Department of Plant Biology Plant Physiology, University of Murcia, E-30100 Murcia, Spain. Corresponding author;

Functional Plant Biology : FPB
|July 22, 2020
PubMed
Summary

Grapevine peroxidases are key enzymes involved in plant defense and cell wall formation. Their activity and hydrogen peroxide (H2O2) levels are modulated by environmental factors, offering insights into plant metabolic regulation.

More Related Videos

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.6K
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
05:17

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

10.6K

Related Experiment Videos

Last Updated: Dec 14, 2025

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

3.0K
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

6.6K
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
05:17

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

10.6K

Area of Science:

  • Plant Biochemistry
  • Enzymology

Background:

  • Peroxidases are heme enzymes catalyzing substrate oxidation using hydrogen peroxide (H2O2).
  • Grapevine peroxidases are polymorphic, thermally stable glycoproteins (35-45 kDa) with characteristic visible spectra.
  • They function in cell walls and vacuoles, influencing cell architecture, lignin deposition, and phenolic metabolism for disease resistance.

Purpose of the Study:

  • To investigate the metabolic regulation of grapevine peroxidases and H2O2 levels under environmental stress.
  • To analyze the specific responses of peroxidase isoenzymes and H2O2 to UV-C radiation and Trichoderma viride elicitors.

Main Methods:

  • Analysis of peroxidase and H2O2 level changes in grapevine cells.
  • Exposure to UV-C radiation and Trichoderma viride elicitors.

Main Results:

  • Both UV-C and T. viride elicitors induced specific alterations in peroxidase isoenzyme and H2O2 levels.
  • T. viride-elicited cells exhibited a unique H2O2 production mechanism, independent of NADPH oxidase-like activities.

Conclusions:

  • The peroxidase-H2O2 system in grapevines is dynamically regulated by environmental stimuli.
  • Grapevine cells provide a model for dissecting the metabolic regulation of peroxidases and H2O2 signaling pathways.