Related Experiment Video
Updated: Aug 2, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
[Comparison between 2 separation methods of IgM and IgG in serologic diagnosis]
M Acetoso1, A Albertoni, G Aureli
1Laboratorio di analisi chimico-cliniche e microbiologiche, Ospedali Riuniti, U.S.L. 3, Pesaro.
Abstract:
The concentrations of the IgG and IgM fractions have been determined on thirty whole sera and after treatment with protein A, or after chromatographic separation. The analysis of the differences has been effected with Student's t test. No statistically significant differences have been noted between the two methods of separation as far as IgM fraction is concerned, but, on the contrary, significant differences are present for the separation of IgG fraction.
More Related Videos
Related Concept Videos
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunocytochemistry and Immunohistochemistry
These...

