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The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations
Gabriela M Pastori1, Alison Huttly1, Jevon West1
1Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Functional Plant Biology : FPB
|July 22, 2020
Summary
The maize Activator/Dissociation (Ac/Ds) transposon system shows functionality in hexaploid wheat. This study demonstrates its effectiveness for potential gene tagging applications in wheat.
Area of Science:
- Plant genetics
- Molecular biology
- Agricultural science
Background:
- The maize Activator/Dissociation (Ac/Ds) transposon system is a key tool for genetic research.
- Its functionality in hexaploid wheat (Triticum aestivum) is crucial for advancing genetic studies in this important crop.
Purpose of the Study:
- To provide background information and evidence for the functionality of the maize Ac/Ds system in hexaploid wheat.
- To assess the potential of the Ac/Ds system for gene tagging in wheat.
Main Methods:
- Crossing transgenic wheat lines carrying immobilised Ac (iAc) and Ds elements.
- Performing transient GUS assays to confirm transposase activity.
- Utilizing PCR and Southern blot analysis to detect Ac, Ds excision, and potential transposition.
Main Results:
- The immobilised Ac transposase demonstrated activity in hexaploid wheat.
- Ds excision occurred in 39% of F1 generation plants.
- Southern analysis suggested potential Ds element transposition events.
Conclusions:
- The maize Ac/Ds system is functional in hexaploid wheat.
- The system shows potential for gene tagging applications in wheat, although further evidence is needed.
- This study validates the Ac/Ds system's effectiveness for wheat genetic research.
