Facile potentiometric sensing of gallic acid in edible plants based on molecularly imprinted polymer

Molecularly imprinted polymers (MIPs) have become a valuable material in the field of electrochemical sensors, due to their selective recognition capabilities towards target molecules. A low-cost potentiometric sensor based on molecular imprinting was developed for the measurement of gallic acid (GA) in edible plants. The imprinted polymer was synthesized by bulk polymerization in the presence of trimethylolpropane triacrylate as the cross-linker and 2,2'-azo-bisisobutyronitrile as the initiator. The sensing component of the sensor was fabricated by the incorporation of MIPs in a polyvinyl chloride matrix. The species and amount of MIPs were optimized, and the imprinted poly(methacrylic acid) sensor was examined and characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and potential response. The proposed sensor exhibited a fast near-Nernst response to GA in the range of 1 × 10^-5 to 3.2 × 10^-4 mol/L. The potentiometric measurement of GA in edible plants was checked by high-performance liquid chromatography, and the two test results showed no significant difference (P > 0.05). The imprinted sensor is applicable to the electrochemical determination of GA in edible plants. PRACTICAL APPLICATION: The proposed MIP-based potentiometric sensor provided a low-cost, efficient, and green tool for the rapid determination of the bioactive ingredient GA in edible plants. The knowledge obtained will offer useful reference to the quality control and bioactive assessment of botanical food.