Fe₃O₄/bioactive glass nanostructure: a promising therapeutic platform for osteosarcoma treatment

An effective strategy of hyperthermia-chemotherapy-regeneration for bone-related cancer treatment is presented. For this purpose, a new approach of magnetic particles (MPs) encapsulated in bioactive glass (BG) structure, with anti-cancer activity, is evaluated. MPs are initially synthesized using a co-precipitation method and then embedded into BG structure through a sol-gel synthesis process. Results confirmed the formation of a crystalline and pure MP structure. MP-BG particles were found to be bioactive by forming a hydroxyapatite layer on their surface. The hyperthermia application of a MP-BG system was also studied. It was found that the particles reach a temperature of 42 °C in an alternating magnetic field. Doxorubicin (DOX), a widely used anticancer drug, was loaded in MP-BG. To enhance the loading efficiency, the BG was surface modified to create NH₂groups on the surface. The encapsulation and release of DOX was studied over 48 h.In vitrotests were performed using human osteosarcoma cell line (MG63). The results demonstrated the non-cytotoxic nature of MP and MP-BG tested at various concentrations. DOX release from MP-BG resulted in decreased MG63 viability. Also, fluorescence microscopy visualization confirmed the intracellular uptake of MP-BG particles and the release of DOX. These results indicate that our suggested strategy of combined hyperthermia-chemotherapy-regeneration using MP-BG structure represents a powerful approach in cancer treatment and tissue regeneration.