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Long non-coding RNA NORAD exhaustion represses prostate cancer progression through inhibiting TRIP13 expression via

Fengling Chen1, Ling Liu2, Shuya Wang2

  • 1Department of Urology, Huaihe Hospital, Henan University, Kaifeng, 475000 Henan China.

Cancer Cell International
|July 23, 2020
PubMed
Abstract

Insights

Long non-coding RNA NORAD and microRNA-495-3p regulate prostate cancer (PCa) by targeting TRIP13. NORAD depletion inhibits PCa progression through the miR-495-3p/TRIP13 pathway, offering a potential treatment strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Prostate cancer (PCa) is a significant health concern for men.
  • Long non-coding RNA activated by DNA damage (NORAD) and microRNA-495-3p (miR-495-3p) are implicated in various cancers.
  • The specific interaction between NORAD and miR-495-3p in PCa remains unelucidated.

Purpose of the Study:

  • To investigate the regulatory mechanism of NORAD and miR-495-3p in prostate cancer.
  • To explore the roles of NORAD and miR-495-3p in PCa cell proliferation, apoptosis, migration, and invasion.
  • To determine the therapeutic potential of targeting the NORAD/miR-495-3p axis in PCa.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis were used to detect gene and protein expression.
  • Cellular assays including MTT, flow cytometry, and transwell assays assessed proliferation, apoptosis, migration, and invasion.
  • Dual-luciferase reporter, RIP, and RNA pull-down assays confirmed molecular interactions.

Main Results:

  • NORAD and TRIP13 were upregulated, while miR-495-3p was downregulated in PCa tissues and cells.
  • NORAD silencing and miR-495-3p upregulation inhibited PCa cell proliferation, migration, and invasion, while promoting apoptosis.
  • NORAD was found to modulate TRIP13 expression by interacting with miR-495-3p, and this axis was crucial for PCa cell behaviors.

Conclusions:

  • NORAD depletion inhibits prostate cancer progression via the miR-495-3p/TRIP13 axis.
  • This regulatory pathway presents a potential therapeutic target for prostate cancer treatment.
Keywords:
NORADProstate cancerTRIP13miR-495-3p

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