Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human monocyte-lymphocyte interaction: a new technique.

P T Fan, D T Yu, C M Pearson

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structure-specific amyloid precipitation in biofluids.

    Nature chemistry·2022
    Same author

    Passive transfer of allergic encephalomyelitis in rats: a tool for drug mechanism studies and detecting late-acting immunosuppressants.

    Inflammopharmacology·2019
    Same author

    Cord blood immune biomarkers in small for gestational age births.

    Journal of developmental origins of health and disease·2014
    Same author

    The effect of some adenosine compounds in pruritus.

    The Lahey Clinic bulletin·2014
    Same author

    Evaluation of quality of life using ASQoL questionnaire in patients with ankylosing spondylitis in a Chinese population.

    Rheumatology international·2006
    Same author

    A neuroprosthesis for high tetraplegia.

    The journal of spinal cord medicine·2001
    Same journal

    Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    Same journal

    Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    Same journal

    FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    Same journal

    An MR1-specific nanobody capable of blocking MR1T cell activation.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    Same journal

    TGF-β controls developmental fate and functional identity of thymic γδ T cells.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    Same journal

    Distinguishing Th17 cells as a unique subset.

    Journal of immunology (Baltimore, Md. : 1950)·2026
    See all related articles

    This study introduces a neuraminidase-galactose oxidase (NGAO) assay to measure monocyte-lymphocyte interactions. The assay reveals NGAO-treated monocytes specifically bind to T lymphocytes, initiating proliferation and indicating early lymphocyte activation.

    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • Understanding monocyte-lymphocyte interactions is crucial for immunology.
    • Existing assays may not fully capture the dynamic physical interactions between these cells.

    Purpose of the Study:

    • To develop and validate a novel rosette-type assay measuring monocyte-lymphocyte physical interaction.
    • To investigate the role of neuraminidase-galactose oxidase (NGAO) in stimulating this interaction and subsequent lymphocyte activation.

    Main Methods:

    • A rosette-type assay was employed using lymphocytes and monocytes treated with neuraminidase-galactose oxidase (NGAO).
    • Monocyte-lymphocyte (ML) rosette formation was quantified under varying conditions (temperature, time, cell ratios, NGAO concentration).
    • Lymphocyte proliferation was assessed via thymidine incorporation and T-lymphocyte subpopulations were analyzed.

    Related Experiment Videos

    Main Results:

    • Neuraminidase-galactose oxidase (NGAO) treatment induced monocyte-lymphocyte (ML) rosette formation and lymphocyte proliferation.
    • Maximal ML rosette formation occurred at 37°C after 4 hours with a 10:1 to 20:1 lymphocyte to monocyte ratio.
    • NGAO-treated monocytes preferentially bound to T lymphocytes and induced proliferation specifically in this subpopulation, correlating with thymidine incorporation.

    Conclusions:

    • The ML rosette assay effectively measures specific physical interactions between monocytes and lymphocytes following NGAO stimulation.
    • This interaction is an early event in lymphocyte activation, directly reflecting the degree of subsequent lymphocyte proliferation.
    • The assay demonstrates NGAO's mitogenic capacity and its selective effect on T lymphocytes.