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GPR50 Distribution in the Mouse Cortex and Hippocampus.

Qifa Li1, Yue Zhang1, Bi-Ying Ge1

  • 1Liaoning Provincial Key Laboratory of Cerebral Diseases, Department of Physiology, Dalian Medical University, Dalian, 116044, People's Republic of China.

Neurochemical Research
|July 23, 2020
PubMed
Summary

G protein-coupled receptor 50 (GPR50) is found in mouse hippocampus and cortex neurons. This GPR50 distribution suggests roles in synaptic plasticity and cognitive functions.

Keywords:
CortexGPR50HippocampusInterneuronPyramidal cells

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • G protein-coupled receptor 50 (GPR50) is homologous to melatonin receptors MT1 and MT2.
  • Previous studies reported GPR50 expression in various brain regions, but its specific distribution in the hippocampus and cortex, and the cell types involved, remained unclear.

Purpose of the Study:

  • To investigate the precise distribution of GPR50 within the adult male mouse hippocampus and cortex.
  • To identify the specific neuronal cell types expressing GPR50 in these brain regions.

Main Methods:

  • Immunofluorescence staining was employed to visualize GPR50 localization in adult male mouse brain sections.
  • Co-localization studies were performed to identify GPR50 expression in specific neuronal subtypes, including those expressing calcium-binding proteins.

Main Results:

  • GPR50 was detected in CA1-3 pyramidal cells and dentate gyrus granule cells of the hippocampus.
  • GPR50 expression was observed in both excitatory and inhibitory neurons, including specific interneuron populations co-expressing calbindin, calretinin, and parvalbumin.
  • Similar GPR50 distribution patterns were found in the cortex.

Conclusions:

  • GPR50 is widely expressed in hippocampal and cortical neurons, including distinct interneuron subtypes.
  • The widespread expression suggests a potential role for GPR50 in regulating synaptic plasticity and cognitive functions.