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Related Experiment Video

Updated: Dec 14, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

A scalable microfluidic chamber array for sample-loss-free and bubble-proof sample compartmentalization by simple

Baobao Lin¹, Zijian Guo, Zhi Geng

¹Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China. pliu@tsinghua.edu.cn.

|July 23, 2020

Summary

A new self-compartmentalization device with capillary burst valves enables efficient sample partitioning for bioassays. This microfluidic platform achieves 100% sample utilization, enhancing applications like digital PCR (dPCR).

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Last Updated: Dec 14, 2025

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Published on: November 23, 2015

A Microfluidic Chip for ICPMS Sample Introduction

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Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

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Published on: October 1, 2007

Area of Science:

Biotechnology
Microfluidics
Analytical Chemistry

Background:

Sample compartmentalization is crucial for bioanalytical techniques like multiplex polymerase chain reaction (PCR) and digital PCR (dPCR).
Existing methods often face challenges with sample utilization and interference from air bubbles.

Purpose of the Study:

To develop a novel self-compartmentalization device for efficient and scalable sample partitioning.
To demonstrate the device's capability in bioassays with high sample utilization.

Main Methods:

Designed a microfluidic device with an array of microchambers connected by a main microchannel.
Incorporated three capillary burst valves (CBVs) for sequential fluid switching and partitioning.
Optimized CBV burst pressures for controlled sample loading and isolation with air or oil.

Main Results:

Achieved a 100% sample utilization rate, unaffected by manual pipetting or air bubbles.
Demonstrated scalability from a few to tens of thousands of microchambers.
Successfully performed mock multiplex loop-mediated isothermal amplifications (LAMP) in a 144-microchamber array.

Conclusions:

The developed self-compartmentalization device offers a robust and versatile platform for sample discretization.
This technology has significant potential for advancing various bioanalytical applications requiring precise sample partitioning.