Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.8K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.8K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.8K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-throughput machine learning-aided antibody discovery for cell surface antigens.

Cell systems·2026
Same author

Efficacy and safety of SENS-501, a dual-AAV otoferlin gene therapy, for DFNB9 congenital deafness.

Molecular therapy. Advances·2026
Same author

Circulating natural killer cells are phenotypically and functionally altered in age-related macular degeneration.

Cell reports. Medicine·2026
Same author

FOXJ1 transcriptional targets in human airway cells and impaired multiciliogenesis in FOXJ1-associated primary ciliary dyskinesia.

American journal of respiratory cell and molecular biology·2026
Same author

Extracellular vesicle-mediated crosstalk in bone: miR-150-5p as a mechanosensitive regulator of osteoclastogenesis.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

A family of ribosome hibernation factors widespread in Archaea.

Nature communications·2026

Related Experiment Video

Updated: Dec 14, 2025

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening
10:01

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

Published on: March 31, 2022

3.6K

SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation.

Oriol Ros1, Sarah Baudet1, Yvrick Zagar1

  • 1Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, 75012 Paris, France.

Cell Reports
|July 23, 2020
PubMed
Summary

Researchers developed SpiCee, a novel genetically encoded calcium chelator. This tool allows precise control over cellular calcium signaling, opening new avenues for research and potential therapeutic applications.

Keywords:
EF handaxon guidancecalciumcalcium buffercalmodulinneuronal migrationparvalbuminsingle-cell pharmacologysubcellular calcium manipulationsubcellular compartment

More Related Videos

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
08:01

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

1.8K
Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

1.5K

Related Experiment Videos

Last Updated: Dec 14, 2025

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening
10:01

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

Published on: March 31, 2022

3.6K
Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
08:01

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

1.8K
Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

1.5K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Neuroscience

Background:

  • Calcium ions (Ca2+) act as critical second messengers in numerous cellular functions.
  • Existing methods for manipulating intracellular calcium lack precise cellular and subcellular specificity.
  • Understanding localized calcium dynamics is essential for cellular process regulation.

Purpose of the Study:

  • To introduce SpiCee, a genetically encoded calcium chelator with tunable affinity.
  • To demonstrate the ability to precisely control and manipulate endogenous calcium signals.
  • To enable investigation of subcellular calcium signaling in vitro and in vivo.

Main Methods:

  • Development of a genetically encoded chelator (SpiCee) with distinct low- and high-affinity calcium-binding sites.
  • Application of SpiCee in single cells for manipulating calcium signaling.
  • In vitro and in vivo testing of SpiCee's efficacy and specificity.

Main Results:

  • SpiCee effectively scavenges calcium ions with controlled affinity.
  • Demonstrated precise alteration of endogenous calcium signaling at the subcellular level.
  • Successful application in both in vitro and in vivo cellular models.

Conclusions:

  • SpiCee offers unprecedented subcellular resolution for manipulating calcium signaling.
  • This technology facilitates the study of localized calcium dynamics in complex biological systems.
  • SpiCee holds potential for therapeutic interventions targeting calcium-mediated diseases.